Home > Sandwich ELISA Kits > Human Ubiquitin carboxyl-terminal hydrolase 1 (USP1) ELISA Kit

Product Name:

Human Ubiquitin carboxyl-terminal hydrolase 1 (USP1) ELISA Kit

Cat.#:

JL17039

Regulatory Status:

RUO

Alternative:

UBP; Ubiquitin Specific Peptidase 1; Ubiquitin Specific Protease 1; Deubiquitinating enzyme 1; Ubiquitin thioesterase 1; Ubiquitin-specific-processing protease 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.083ng/mL

Specificity:

This kit recognizes Human USP1 in samples. No significant cross-reactivity or interference between Human USP1 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Cell Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| USP1

Ubiquitin carboxyl-terminal hydrolase 1 is an enzyme that is encoded by the USP1 gene.This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.45	1.46	0.89	0.57	0.39	0.28	0.23	0.09
Corrected OD	2.36	1.37	0.8	0.48	0.3	0.19	0.14	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human USP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	95-105	102
Cell Culture Supernates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human USP1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	86-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	110